SPY650-FastAct™ is a bright, far red, fluorogenic & non toxic F-actin stain based on our SPY™ dyes series. Its optimized structure allows to label F-actin in live cells with high specificity and low background. The unique and unmatched feature of SPY650-FastAct™ is its ability to label very dynamic actin, like LifeAct™** but the probe does not require any genetic manipulation, transfection or overexpression of fluorescent proteins. SPY650-FastAct™ is based on our bright, photostable & far red SPY650 fluorophore which is far superior than fluorescent proteins.
SPY650-FastAct™ enables multicolor imaging with SPY505, SPY555, SPY595, SPY700, GFP or m-cherry. SPY650-FastAct™ can be imaged with standard Cy5 filterset. It can be used for widefield, confocal, SIM or STED imaging of living cells and tissue. Probe quantity allows 100 staining experiments.*
|Absorbance maximum λabs||652 nm|
|Fluorescence maximum λfl||674 nm|
|Works on fixed cells?||No|
|Probe quantity||100 stainings*|
|Fluorescence lifetime||3.0 ns|
|STED depletion wavelength||775 nm|
*Based on the following conditions: 0.5 ml staining solution / staining experiment with 1x probe concentration. The number of staining experiments can be further increased by reducing the staining solution volume or the probe concentration.
**LifeAct™ is a registered trademark of Ibidi GmbH.