3T3 cells stained with SiR700-DNA (widefield)3T3 cells stained with SiR700-DNA (widefield)

SiR700-DNA kit

CHF260.00

SiR700-DNA is a fluorogenic, cell permeable and highly specific probe for DNA

Kit contains 35 nmol SiR700-DNA and 1 umol verapamil

Price excludes VAT and shipping

Customers from North America (US and CA), please order from our dedicated supplier Cytoskeleton, Inc.

Customers from Europe (without Switzerland), please order from our dedicated supplier tebu-bio

cat#: SC015 Categories: , ,

Description

SiR700-DNA is based on the DNA minor groove binder bisbenzimide, it allows the labelling of DNA in live cells with high specificity and low background. SiR700-DNA is fluorogenic, cell permeable and highly specific for DNA. Sir700-DNA stains the nuclei of live cells without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SiR700-DNA is compatible with GFP and/or m-cherry fluorescent proteins and is a non cytotoxic alternative to DRAQ5. It can be imaged with standard Cy5 filtersets. SiR700-DNA can be used for widefield, confocal, SIM or STED imaging in living cells and tissue. Probe quantity allows 50 – 200 staining experiments.*

In combination with standard SiR-probes, SiR700-DNA allows dual colour imaging experiments in live cells. For more information please visit www.spirochrome.com/dualcolour.

Optical properties

λabs 682 nm
εmax 1.0·105 mol-1·cm-1
λfl 716 nm
Recommended STED depletion line: 810 nm

 

 

*Based on the following conditions: 0.5 – 1 ml staining solution / staining experiments with 0.5 – 1 uM probe concentration. The number of staining experiments can be further increased by reducing volume or probe concentration.

SiR700-DNA kit datasheet

Verapamil usage guidelines

SiR700-DNA MSDS

Ex/Em spectra (excel format)

1. How many experiments can I do with 35 nmol of SiR700-DNA?
Depending on the sample volume and the concentration used for labelling, about 35-150 experiments can be done with one vial. 
2. Is SiR700-DNA stable at room temperature?
Yes, the probe is stable at room temperature for a few days. However, it is recommended to store SiR700-DNA or solutions thereof at -20°C to maximize shelf-life. 
3. Does SiR700-DNA stain fixed cells?
Yes, SiR700-DNA can be used with methanol or PFA fixed cells. However, the mounting medium has a strong influence on the brightness of SiR700-DNA. While cells mounted with PBS or Vectashield® gave a low signal, cells mounted with pure glycerol had very bright nuclei. We therefore recommend to use pure glycerol as mounting medium when staining fixed cells with SiR700-DNA.
4. Is SiR700-DNA toxic to the cells?
SiR700-DNA is based on the DNA minor groove binding molecule bisbenzimide. It can therefore modify DNA metabolism in living cells. Mitotic duration and chromosome missegregation remained unchanged at concentrations up to 10 uM SiR-DNA. However, an independent study1) using Cyclin B1 and γH2AX reporter assays recommended to use concentrations equal or below 250 nM SiR-DNA if long term (>12h) imaging experiments are planned. By analogy we recommend to use the same concentration of SiR700-DNA for long term (>12h) imaging experiments. For all other purposes, using 1-3 uM SiR700-DNA for staining is recommended.
1) Sen, Onur, Adrian T. Saurin, and Jonathan MG Higgins.; Scientific reports 8.1 (2018): 7898. 
5. Which organisms and tissues are stained by Sir700-DNA?
This list is only reporting known working cell lines, tissues or organisms. If a cell line, tissue or organism is not present in the list, it does not mean that it will not be stained by SiR-Probes.

Homo sapiens : U2OS, fibroblasts, HeLa MCF-10A, A549, erythrocytes.

Drosophila melanogaster : Notum epithelium, S2.

Help us to complete the list by reporting your positive experiences! 

6. Do SiR-probes work in 3D cell cultures?
Yes, the probes are able to stain cells in a 3D growth environment.
7. Why are your prices displayed only in CHF (Swiss Francs)?
Our company is based in Switzerland and therefore our list prices are displayed in CHF (Swiss Francs). Upon request, we will provide you with a binding quote (valid 1 month) based on the displayed CHF prices and the actual exchange rate. Please indicate your preferred currency (USD, EUR, CHF) and payment method (bank transfer OR credit card via PayPal).
8. Where can I order my Spirochrome products?
Spirochrome has appointed dedicated distributors for the SiR-probes: SiR-actin, SiR-tubulin, & cytoskeleton-kit and SiR700-DNA. Customers from North America (US and CA) can order them through Cytoskeleton, Inc. and European customers (without Switzerland) can order them through tebu-bio.
The reagents: SiR-COOH, SiR-NHS, & Boc-Lys-jasplakinolide, can be ordered through our website via email or the webshop.

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