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3T3 cells stained with SiR700-DNA (widefield)
3T3 cells stained with SiR700-DNA (widefield)
3T3 cells stained with SiR700-DNA (widefield)
3T3 cells stained with SiR700-DNA (widefield)

SiR700-DNA kit

SiR700-DNA is a fluorogenic, cell permeable and highly specific probe for DNA

Kit contains 35 nmol SiR700-DNA and 1 umol verapamil


SKU: SC015 Category: SiR700 Probes
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Product Description

SiR700-DNA is based on the DNA minor groove binder bisbenzimide, it allows the labelling of DNA in live cells with high specificity and low background. SiR700-DNA is fluorogenic, cell permeable and highly specific for DNA. SiR700-DNA stains the nuclei of live cells without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SiR700-DNA is compatible with GFP and/or m-cherry fluorescent proteins and is a non cytotoxic alternative to DRAQ5. It can be imaged with standard Cy5 filtersets. SiR700-DNA can be used for widefield, confocal, SIM or STED imaging in living cells and tissue. Probe quantity allows 50 – 200 staining experiments.*

In combination with standard SiR-probes, SiR700-DNA allows dual colour imaging experiments in live cells. For more information please visit www.spirochrome.com/dualcolour.

Optical properties

λabs 682 nm
εmax 1.0·105 mol-1·cm-1
λfl 716 nm
Recommended STED depletion line: 810 nm

*Based on the following conditions: 0.5 – 1 ml staining solution / staining experiments with 0.5 – 1 uM probe concentration. The number of staining experiments can be further increased by reducing volume or probe concentration.

Latest citing articles

SiR700-DNA kit datasheet

Verapamil usage guidelines

SiR700-DNA SDS

Ex/Em spectra (excel format)

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