Mito Flipper-TR® is a fluorescent probe that specifically targets the membrane of mitochondria of cells and reports membrane tension changes through its fluorescence lifetime changes. It contains a mitochondria targeting motive as well as a Flipper fluorophore1-2) which senses changes of the organization of lipid bilayer membranes through changes of the twist angle and polarization between the two twisted dithienothiophenes of the mechanophore. Mito Flipper-TR® spontaneously labels mitochondria of live cells and is only fluorescent when inserted in a lipid membrane. It has a broad absorption and emission spectrum, excitation can be commonly performed with a 488nm laser, while emission is collected between 575 and 625nm. A detailed protocol for staining and FLIM imaging can be found in the datasheet. Probe quantity allows 35 – 150 staining experiments.*
λabs 480 nm
λfl 600 nm
εmax 1.66·104 mol-1·cm-1 (in DMSO)
lifetime 2.8-7 ns
QY 30% (in AcOEt)
Flipper-TR® fluorophore Abs/Em spectra (Excel format)
1) Colom A, et al: A fluorescent membrane tension probe, Nat Chem, 2018, 10:1118–1125 ().
2) Goujon A, et al: Mechanosensitive Fluorescent Probes to Image Membrane Tension in Mitochondria, Endoplasmic Reticulum, and Lysosomes, J. Am. Chem. Soc. 141, 8, 3380–3384, 2019.
*Based on the following conditions: 0.5 – 1 ml staining solution / staining experiments with 0.5 – 1 uM probe concentration. The number of staining experiments can be further increased by reducing volume or probe concentration.
Mito Flipper-TR® probe is distributed by Spirochrome under an exclusive license from the University of Geneva, Switzerland and was developed by the NCCR Chemical Biology.