News & updates
2 October 2020
The new organelle specific mechanosensitive Flipper-TR™ probes are available!
We introduce new Flipper-TR™ probes that target ER, mitochondria & lysosomes of live cells. Those were developped by NCCR Chemical Biology groups of Aurelien Roux and Stefan Matile in a recent article1)
1. “Mechanosensitive Fluorescent Probes to Image Membrane Tension in Mitochondria, Endoplasmic Reticulum, and Lysosomes”; Antoine Goujon, et. al.;J. Am. Chem. Soc. 141, 8, 3380–3384, 2019.
live Cos7 cells labeled with ER Flipper-TR™. Courtesy of N. Schilling, D. Haenni & U. Ziegler; Center for Microscopy and Image Analysis; University of Zurich Switzerland.
FLIM image of live Cos7 cells labeled with Mito Flipper-TR™. Courtesy of N. Schilling, D. Haenni & U. Ziegler; Center for Microscopy and Image Analysis; University of Zurich Switzerland.
22 September 2020
Our new SPY620 probes are here!
We are proud to introduce our new red emitting SPY™ probes based on Map618 fluorophore developed by the Johnsson group in Heidelberg.1)
SPY620-actin is a highly fluorogenic probe for F-actin with a turn-on ratio above 400 fold upon actin binding.
SPY620-DNA is a bright probe for nuclear staining.
Both DNA and actin probes work on live and fixed cells.
We also introduce the SNAP-tag substrate SPY620-BG for SNAP-tag* labeling inside living cells.
1. “A general strategy to develop cell permeable and fluorogenic probes for multicolour nanoscopy”; Lu Wang, et. al.; Nature Chemistry 12, 165-172, 2020.
Image: live Cos7 cells labeled with SPY620-actin. Courtesy of N. Schilling, D. Haenni & U. Ziegler; Center for Microscopy and Image Analysis; University of Zurich Switzerland.
*SNAP-tag™ is a registered trademark of New England Biolabs, Inc.
16 July 2020
Coming soon: SPY620 probes
Spirochrome will soon introduce new red emitting SPY™ probes based on the Map618 fluorophore developed in the Johnsson group in Heidelberg.1)
SPY620-actin for F-actin and SPY620-DNA for nucleus staining as well as SPY620-BG for SNAP-tag labeling will be soon available!
1. “A general strategy to develop cell permeable and fluorogenic probes for multicolour nanoscopy”; Lu Wang, et. al.; Nature Chemistry 12, 165-172, 2020.
Rat primary hippocampal neurons stained with SPY620-actin showing showing actin periodic structures. Image source: figure 4d of 1)
9 July 2020
The Flipper-TR family expands!
Spirochrome will soon introduce new Flipper-TR™ probes that target ER, mitochondria & lysosomes within live cells. Those were previously introduced by NCCR Chemical Biology groups of Aurelien Roux and Stefan Matile in a recent article1)
1. “Mechanosensitive Fluorescent Probes to Image Membrane Tension in Mitochondria, Endoplasmic Reticulum, and Lysosomes”; Antoine Goujon, et. al.;J. Am. Chem. Soc. 141, 8, 3380–3384, 2019.
19 June 2020
Spirochrome now provides highly cell permeable benzylguanine (BG) derivatives of SiR and SPY™ dyes . BG derivatives are substrates to the self-labeling tag SNAP-tag™*.
Our new BG derivatives of SiR and SPY™ fluorophores are live cell compatible substrates for SNAP-tag™* bearing fluorophores with high brightness, high photostability which are compatible with superresolution microscopy (STED & SIM) 1-3).
SPY555-BG, SiR650-BG and SiR700-BG are available.
1. “A general strategy to develop cell permeable and fluorogenic probes for multicolour nanoscopy”; Lu Wang, et. al.; Nature Chemistry 12, 165-172, 2020.
2. “A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins”; Nature Chemistry 5, 132–139, 2013.
3. “Fluorogenic Probes for Multicolor Imaging in Living Cells”; J. Am. Chem. Soc.,138, 30, 9365–9368, 2016
*SNAP-tag™ is a registered trademark of New England Biolabs, Inc.
10 June 2020
Spirochrome will soon provide highly cell permeable benzylguanine (BG) derivatives of SiR and SPY™ dyes . BG derivatives are substrates to the self-labeling tag SNAP-tag™*. BG derivatives of SiR and SPY™ fluorophores are live cell compatible substrates for SNAP-tag™* bearing fluorophores with high brightness, high photostability which are compatible with superresolution microscopy (STED & SIM).
*SNAP-tag™ is a registered trademark of New England Biolabs, Inc.
13 May 2020
Our SPY™ probes family for live cell imaging will expand soon! A new SPY™ fluorophore will fill the gap between SPY595 and SPY650 probes, providing bright and highly fluorogenic probes!
Stay excited!
16 April 2020
Our new SPY™ probes for live cell imaging of nucleus, actin and microtubules are available. 5 different emission wavelengths are available including FITC, TMR, Alexa™594, Cy5 and Cy5.5. The new SPY™ probes are based on new fluorophores that possess high photostability, high brightness and an excellent compatibility with STED and SIM superresolution imaging.
Find our products here: SPY™ probes
06 December 2019
A new landmark paper from the Kai Johnsson group in Heidelberg was published in Nature Chemistry. A revolutionary concept to convert green, orange or red rhodamine-like fluorophores into well behaved cell permeable, fluorogenic, superresolution compatible probes, just like our well established SiR-probes.
This breakthrough is based on acidic spiroamides as analogues of the SiR fluorophore spirolactone that makes SiR so special.
Spirochrome is the exclusive licensee of this technology and a whole suite of new products will be introduced under the brand SPY™ in early 2020.
28 November 2019
Welcome to the Spiro-team Wanda!
Spirochrome is now a proud sponsor of Wanda which is living in a donkey sanctuary near the company’s headquarter! We will update you with our new mascot adventures.
Wanda is taken very good care of and is living happily with seventeen other donkeys that have been rescued from abuse, neglect, malnourishment or abandonment.
This is the website (in German) of her sweet home:
http://www.pferdehof-gruenegg.ch/
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04 August 2019
Happy 5th anniversary !
It all started on August 04 2014, when Spirochrome’s first order was shipped to Heidelberg, Germany! The last 5 years were an amazing journey where the whole Spiro-team worked very hard to provide bright, innovative and reliable probes with the best quality through an excellent service. Our whole team is very proud of its achievements.
With these lines we take the opportunity to thank all the scientists who trusted our SiR-probes and did great Science with them. We enjoyed reading every single paper or study using our probes in ways we couldn’t even think of! We are convinced that more great stories will be reported soon with the help of our products.
To celebrate our anniversary, we will soon introduce a whole suite of new fluorogenic live cell probes working in the green, orange, red and far red channels: the SPY-probes. Follow our Twitter account to SPY on us!
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06 April 2019
Kai Johnsson’s lab, in collaboration with Spirochrome, developed a new concept of photoactivatable fluorophore for single molecule localization microscopy in live cells: PA-SiR.
The preprint is online on BioRxiv
PA-SiR fluorescence can be turned on by UV-light (e.g. a 405 nm laser), forming a bright silicon rhodamine fluorophore emitting in the far-red. The mechanism of photoconversion is unprecedented and involves a photoinduced proton transfer. As the photoconversion is intrinsic to the molecule, no caging group is needed to switch to the fluorescent form, making the fluorophore cell permeable and bio-compatible.
02 April 2019
Our live cell actin probe SiR-actin took a space flight in the International Space Station (ISS). Cora Thiel et al. built a miniaturized SIM microscope and imaged live and fixed cells stained with SiR-actin in the ISS. Congratulations to the authors.
Read the article here
04 March 2019
Our fluorescent membrane tension probe Flipper-TR® is now available at our distributors Cytoskeleton, Inc (north America) and Tebu-bio (Europe without CH).
23 January 2019
Spirochrome is proud to support the 11th Conference of the International PhD Program in Life Sciences.
The conference, organized by graduate students, is designed to initiate and promote scientific discussion and networking within the Swiss and wider life sciences community.
07 January 2019
Happy New year!
Spirochrome wishes you a happy and very successful 2019.
Follow us on Twitter and find out the latest information about our products and the coming ones.
2019 will be colorful!
27 November 2018
Flipper-TR: the first fluorescent live cell fluorescent membrane tension probe is now available from Spirochrome
06 January 2017
Spirochorme introduces the new SiR-reagent SiR-BCN for click chemistry with tetrazine and azide derivatives.
05 September 2016
Spirochrome proudly launches the brand new and further red emitting SiR700 probes for actin, DNA and microtubules. SiR700 probes allow dual colour imaging experiments in live cells and dual colour superresolution imaging with STED and SIM. In addition, two new fluorogenic live cell probes for lysosomes are introduced: SiR-lysosome and SiR700-lysosome. The probes were published by the Johnsson group in JACS: http://pubs.acs.org/doi/abs/10.1021/jacs.6b04782.
26 July 2016
Soon available at Spirochrome: A new SiR-probe for lysosomes and four brand new red shifted SiR-probes for actin, tubulin, DNA and lysosomes for dual colour imaging in live cells: http://pubs.acs.org/doi/abs/10.1021/jacs.6b04782
22 July 2016
The European Patent Office grants the patent based on the patent application WO2013029650 covering carboxylated silicon rhodamine derivatives and probes within the European territory, of which Spirochrome is the exclusive licensee.
4 May 2016
The US patent office grants the US patent US9346957 covering on the US territory carboxylated Silicon Rhodamine derivatives. Spirochrome is the exclusive licensee.
5 October 2015
Spirochrome further expands its products portfolio and launches three new SiR-reagents: SiR-alkyne, SiR-azide and SiR-tetrazine. These reagents allow bioortogonal click chemistry.
1 October 2015
Spirochrome launched its new probe SiR-DNA. SiR-DNA is a far-red, cell permeable, non-toxic probe for DNA.
25 May 2015
Spirochrome has appointed Cytoskeleton, Inc. as its exclusive distributor for the probes SiR-actin kit, SiR-tubulin kit and Cytoskeleton kit in North America (US and CA). Customers from these countries can now find and order our probes from Cytoskeleton, Inc. here
12 Feruary 2015
Since February 2015, Verapamil, an efflux pump inhibitor, is included free of charge with every order of SiR-actin, SiR-tubulin or Cytoskeleton kit. Verapamil has been sown to enhace the uptake of SiR-probes on living cells Read more. The products SC001 SiR-actin and SC002 SiR-tubulin have therfore been renamed “SiR-actin kit” and “SiR-tubulin kit”.
19 January 2015
Leica and Spirochrome released the application note “A Bright Dye for Live-Cell STED Microscopy” exemplifying the enormous power of the combination of SiR-based probes and STED nanoscopy.
July 2014
Spirochrome was granted by EPFL an exclusive license from the patent application WO2013029650 to exclusively commercialize Silicon Rhodamine derivatives and probes.