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STED image of Live HeLa cell stained with SPY555-tubulin (magenta), SPY595-DNA (orange) and SiR-actin (white) with a single 775 nm STED line
HeLa cell stained with 1x SPY555-tubulin
HeLa cells stained with 1x SPY555-tubulin
STED image of Live HeLa cell stained with SPY555-tubulin (magenta), SPY595-DNA (orange) and SiR-actin (white) with a single 775 nm STED line
HeLa cell stained with 1x SPY555-tubulin
HeLa cells stained with 1x SPY555-tubulin

SPY555-tubulin

SPY555-tubulin is a bright orange, fluorogenic, non toxic, cell permeable and highly specific live cell probe for fluorescence imaging of microtubules.

Product contains 1 vial of SPY555-tubulin in lyophilised form allowing to prepare 50 ul of 1000x DMSO stock solution (100 stainings*).


SKU: SC203 Category: SPY probes
  • Description
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Product Description

SPY555-tubulin is a bright & non toxic live cell microtubule stain based on our SPYTM dyes series. Its optimized structure allows quick labeling of microtubules in live cells with high specificity and very low background. SPY555-tubulin stains microtubules in live cells without the need for genetic manipulation or overexpression of fluorescent proteins. Its absorbance and emission spectra are similar to tetramethylrhodamine (TMR). SPY555-tubulin enables multicolor imaging with SPY505, SPY595, SPY650, SPY700, SiR and GFP. SPY555-tubulin can be imaged with standard TMR or Cy3 filtersets. It can be used for widefield, confocal, SIM or STED imaging in living cells and tissue. Probe quantity allows 100 staining experiments.*

Probe Properties

 

Absorbance maximum λabs 555 nm
Fluorescence maximum λfl 580 nm
Works on fixed cells? No
Probe quantity 100 stainings*
Fluorescence lifetime 2.4 ns
STED depletion wavelength 660 or 775 nm
Shipping room temperature
Storage -20°C

 

*Based on the following conditions: 0.5 ml staining solution / staining experiment with 1x probe concentration. The number of staining experiments can be further increased by reducing the staining solution volume or the probe concentration.

Latest citing articles

SPY555-tubulin datasheet

SPY555-tubulin SDS

Ex/Em spectra (excel format)

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