SPY555-tubulin is a bright & non toxic live cell microtubule stain based on our SPYTM dyes series. Its optimized structure allows quick labeling of microtubules in live cells with high specificity and very low background. SPY555-tubulin stains microtubules in live cells without the need for genetic manipulation or overexpression of fluorescent proteins. Its absorbance and emission spectra are similar to tetramethylrhodamine (TMR). SPY555-tubulin enables multicolor imaging with SPY505, SPY595, SPY650, SPY700, SiR and GFP. SPY555-tubulin can be imaged with standard TMR or Cy3 filtersets. It can be used for widefield, confocal, SIM or STED imaging in living cells and tissue. Probe quantity allows 100 staining experiments.*
|Absorbance maximum λabs||555 nm|
|Fluorescence maximum λfl||580 nm|
|Works on fixed cells?||No|
|Probe quantity||100 stainings*|
|Fluorescence lifetime||2.4 ns|
|STED depletion wavelength||660 or 775 nm|
*Based on the following conditions: 0.5 ml staining solution / staining experiment with 1x probe concentration. The number of staining experiments can be further increased by reducing the staining solution volume or the probe concentration.