Product Description
PKmem 555 is a bright, non-phototoxic & non-toxic plasma membrane probe based on the PKmem™ dyes developed by the lab of Zhixing Chen at Peking University1). PKmem 555 labels the plasma membrane in live cells with very high specificity. The unique and unmatched feature of PKmem 555 is its extremely low phototoxicity, due to the presence of the intramolecular triplet quencher cyclooctatetraene (COT) group. It allows to perform long term live cell imaging of the plasma membrane without damaging the cells. PKmem 555 does not require any genetic manipulation, transfection or overexpression of fluorescent proteins. PKmem 555 enables multicolor imaging with SPY505, SPY595, SPY650, SPY700, SiR or GFP. PKmem 555 can be imaged with a standard TMR or Cy3 filterset. It can be used for widefield, confocal, or SIM imaging in living cells and tissue. Contains 1 vial of PKmem 555 (lyophilized).
Probe Properties
| Absorbance maximum λabs | 556 nm |
| Fluorescence maximum λfl | 572 nm |
| Works on fixed cells? | Yes, PFA or GA |
| Probe quantity | 100 stainings* |
| Fluorescence lifetime | n.d. |
| STED depletion wavelength | 660 nm |
| Shipping | room temperature |
| Storage | -20°C |
*Based on the following conditions: 0.5 ml staining solution / staining experiment with 1x probe concentration. The number of staining experiments can be further increased by reducing the staining solution volume or the probe concentration.
1. Jing Ling et al. “A gentle palette of plasma membrane dyes”, BioRxiv, 2024
