Product Description
SiR650-BG is the benzylguanine derivative of our SiR fluorophore. It emits light in the far-red, it is fluorogenic, highly cell permeable and well suited for STED and SIM superresolution imaging. SiR650-BG can be imaged with a standard Cy5 filterset. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. It has the same structure as SNAP-Cell® 647-SiR and also identical to SiR-SNAP, the name used in its original publication in Nature Chemistry1).
Benzylguanine (BG) is the substrate of the self labeling tag SNAP-tag™*. Upon reaction with a BG derivative, SNAP-tag™* forms a covalent bond with the substrate and releases guanine. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as SNAP-tag™* fusion.
Probe Properties
| Absorbance maximum λabs | 652 nm |
| Fluorescence maximum λfl | 674 nm |
| Works on fixed cells? | Yes, any type of fixation method |
| Probe quantity | 35 nmol |
| Fluorescence lifetime | 3.0 ns |
| MW | 725.9 g/mol |
| STED depletion wavelength | 775 nm |
| Shipping | room temperature |
| Storage | -20°C |
1. “A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins”; Nature Chemistry 5, 132–139, 2013.
*SNAP-tag™ is a registered trademark of New England Biolabs, Inc.
