SiR650-BG is the benzylguanine derivative of our SiR fluorophore. It emits light in the far-red, it is fluorogenic, highly cell permeable and well suited for STED and SIM superresolution imaging. SiR650-BG can be imaged with a standard Cy5 filterset. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. It has the same structure as SNAP-Cell® 647-SiR and also identical to SiR-SNAP, the name used in its original publication in Nature Chemistry1).
Benzylguanine (BG) is the substrate of the self labeling tag SNAP-tag™*. Upon reaction with a BG derivative, SNAP-tag™* forms a covalent bond with the substrate and releases guanine. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as SNAP-tag™* fusion.
|Absorbance maximum λabs||652 nm|
|Fluorescence maximum λfl||674 nm|
|Works on fixed cells?||Yes, any type of fixation method|
|Probe quantity||35 nmol|
|Fluorescence lifetime||3.0 ns|
|STED depletion wavelength||775 nm|
1. “A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins”; Nature Chemistry 5, 132–139, 2013.
*SNAP-tag™ is a registered trademark of New England Biolabs, Inc.