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excitation and emission plot of SiR
STED image of Live HeLa cell stained with SPY555-tubulin (magenta), SPY595-DNA (orange) and SiR-actin (white) with a single 775 nm STED line
Drosophila hind gut stained with SiR-actin
MCF10A cells (3D culture) stained with SiR-actin
excitation and emission plot of SiR
STED image of Live HeLa cell stained with SPY555-tubulin (magenta), SPY595-DNA (orange) and SiR-actin (white) with a single 775 nm STED line
Drosophila hind gut stained with SiR-actin
MCF10A cells (3D culture) stained with SiR-actin

SiR-actin kit

SiR-actin is a fluorogenic, cell permeable and highly specific probe for F-actin

Kit contains 50 nmol SiR-actin and 1 umol verapamil


SKU: SC001 Category: SiR probes
  • Description
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Product Description

SiR-actin is based on the F-actin binding natural product jasplakinolide. SiR-actin is fluorogenic, cell permeable and highly specific for F-actin. Sir-actin stains endogenous F-actin without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SiR-actin is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with standard Cy5 filtersets. SiR-actin can be used for widefield, confocal, SIM or STED imaging in living cells and tissue. Probe quantity allows 50 – 200 staining experiments.*

Optical properties

λabs 652 nm
εmax 1.0·105 mol-1·cm-1
λfl 674 nm

*Based on the following conditions: 0.5 – 1 ml staining solution / staining experiments with 0.5 – 1 uM probe concentration. The number of staining experiments can be further increased by reducing volume or probe concentration.

Latest citing articles

SiR-actin kit datasheet

Verapamil usage guidelines

SiR-actin SDS

Ex/Em spectra (excel format)

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  • SiR-DNA kit

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  • SiR-lysosome kit

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