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SiR620 Absorption and Emission Spectra
SiR620 Absorption and Emission Spectra

SPY620-BG

SPY620-BG is a bright, cell permeable and red emitting benzylguanine substrate for labeling SNAP-tag™* in cellular fluorescence imaging experiments.

Product contains 1 vial of SPY620-BG (35 nmol) in lyophilised form allowing to prepare 35 ul of 400x DMSO stock solution.

SKU: SC404 Category: Fluorophore & Reagents
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Product Description

SPY620-BG is the benzylguanine derivative of our SPY620 fluorophore. It emits light in the orange part of the UV-ViS spectrum, it is fluorogenic, highly cell permeable and well suited for STED and SIM superresolution imaging. SPY620-BG can be imaged with e.g. using 610/20 nm and 650/40 nm excitation and emission filters respectively. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. It has a structure similar to MaP618 fluorophore the name used in its original publication in Nature Chemistry1).

Benzylguanine (BG) is the substrate of the self labeling tag SNAP-tag™*. Upon reaction with a BG derivative, SNAP-tag™* forms a covalent bond with the substrate and releases guanine. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as SNAP-tag™* fusion.

Probe Properties

 

Absorbance maximum λabs 619 nm
Fluorescence maximum λfl 636 nm
Works on fixed cells? Yes, any type of fixation method
Probe quantity 35 nmol
Fluorescence lifetime 2.9 ns
STED depletion wavelength 775 nm
Shipping room temperature
Storage -20°C

 

1. “A general strategy to develop cell permeable and fluorogenic probes for multicolour nanoscopy”; Lu Wang, et. al.; Nature Chemistry 12, 165-172, 2020.

*SNAP-tag™ ia a registered trademark of New England Biolabs, Inc.

SPY620-BG datasheet

SPY620-BG SDS

Ex/Em spectra (excel format)

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