SPY555-DNA is a bright & non toxic live cell nuclear stain based on our SPYTM dyes series. Its optimized structure allows quick labeling of DNA in live & fixed cells with high specificity and very low background. SPY555-DNA stains the nuclei of live or fixed cells without the need for genetic manipulation or overexpression of fluorescent proteins. Its absorbance and emission are similar to tetramethyl-rhodamine (TMR). SPY555-DNA enables multicolor imaging with SPY505, SPY595, SPY650, SPY700, SIR and GFP. SPY555-DNA can be imaged with standard TMR or Cy3 filtersets. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. Probe quantity allows 100 staining experiments.*
|Absorbance maximum λabs||555 nm|
|Fluorescence maximum λfl||580 nm|
|Works on fixed cells?||yes, PFA and methanol|
|Probe quantity||100 stainings*|
|Fluorescence lifetime||2.4 ns|
|STED depletion wavelength||660 or 775 nm|
*Based on the following conditions: 0.5 ml staining solution / staining experiment with 1x probe concentration. The number of staining experiments can be further increased by reducing the staining solution volume or the probe concentration.