SPY700-FastAct™ is a bright, far red, fluorogenic & non toxic F-actin stain based on our SPY™ dyes series. Its optimized structure allows to label F-actin in live cells with high specificity and low background. The unique and unmatched feature of SPY700-FastAct™ is its ability to label very dynamic actin, like LifeAct™** but the probe does not require any genetic manipulation, transfection or overexpression of fluorescent proteins. SPY700-FastAct™ is based on our bright, photostable & far red SPY700 fluorophore which is far superior than fluorescent proteins.
SPY700-FastAct™ enables multicolor imaging with SPY505, SPY555, SPY595, SPY650, GFP or m-cherry. SPY700-FastAct™ can be imaged with standard Cy5 filterset. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. Probe quantity allows 100 staining experiments.*
|Absorbance maximum λabs||696 nm|
|Fluorescence maximum λfl||718 nm|
|Works on fixed cells?||Yes, PFA fixed cells|
|Probe quantity||100 stainings*|
|Fluorescence lifetime||2.2 ns|
|STED depletion wavelength||780-820 nm|
*Based on the following conditions: 0.5 ml staining solution / staining experiment with 1x probe concentration. The number of staining experiments can be further increased by reducing the staining solution volume or the probe concentration.
**LifeAct™ is a registered trademark of Ibidi GmbH.