Our technology is based on silicon rhodamine (SiR) and SPY™ fluorophores1,3). These are bright and photostable rhodamine-like dye. Their key features are cell permeability, high photostability, fluorogenic character and compatibility with super-resolution microscopy. The fluorescence excitation and emission of SiR and SPY™ fluorophores cover the whole visible spectrum. SiR and SPY™ fluorophores are compatible with most microscopes usually falling within usual settings such as Cy3, Cy5 or FITC. We use our SiR and SPY™ fluorophores in conjunction with ligands binding to cellular structures such as DNA, actin, microtubules or lysosomes to create reliable and high quality probes for live cell imaging. This combination set SiR and SPY™ based probes at the leading edge of high performance & high quality fluorescent probes.
- 1. Fluorogenic probes for live-cell imaging of the cytoskeleton, G. Lukinavičius et al., Nature Methods, 11, 731–733 (2014)
- 2. A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins G. Lukinavičius et al., Nature Chemistry, 5, 132–139 (2013).
- 3. A general strategy to develop cell permeable and fluorogenic probes for multicolour nanoscopy Lu Wang et al., Nature Chemistry, 12, 165–172 (2020).