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excitation and emission plot of SiR
excitation and emission plot of SiR

SiR-BCN

SiR-BCN is a clickable derivative of SiR. It reacts with tetrazine or azide derivatives and allows the preparation of custom SiR-conjugates.

Quantity: 1mg

SKU: SC017 Category: Fluorophore & Reagents
  • Description
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Product Description

SiR-BCN is a bicyclo[6.1.0]nonyne (BCN) derivative of the fluorophore silicon rhodamine (SiR). SiR-BCN contains a short PEG linker between the SiR fluorophore and the BCN moiety. SiR-BCN reacts quickly with tetrazine and azide derivatives to form covalent linkages. Small molecules, peptides, oligonucleotides or proteins bearing the appropriate tetrazine or azide can be conjugated to SiR-BCN. SiR excitation and emission are compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with a standard Cy5 filterset.

Properties

λabs 652 nm
εmax 1.0·105 mol-1·cm-1
λfl 674 nm
MW =779.0 g/mol
CF260 = n.d.
CF280 = n.d.

References

1. A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins; G. Lukinavičius et al.; Nature Chemistry; 5, 132–139 (2013).

SiR-BCN datasheet

SiR-BCN SDS

Ex/Em spectra (excel format)

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