Description
SiR-tetrazine is a clickable derivative of SiR. This probe reacts with dienophiles such as trans-cyclooctene (TCO) or bicyclononyne (BCN) derivatives, enabling the preparation of custom SiR probes and conjugates. SiR-tetrazine is particularly useful in combination with unnatural amino acid (UAA) incorporation to prepare site selective protein conjugates. SiR-tetrazine is cell permeable and fluorogenic, it can be used to stain live cells as well as fixed ones. SiR is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with a standard Cy5 filterset.
Properties
λabs 652 nm
εmax 1.0·105 mol-1·cm-1
λfl 674 nm
MW =641.8 g/mol
CF260 = 0.116
CF280 = 0.147
References
1. A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins; G. Lukinavičius et al.; Nature Chemistry; 5, 132–139 (2013).
2. Superresolution imaging of the Golgi in live cells with a bioorthogonal ceramide probe;R. S. Erdmann et al.; Angew. Chem. Int. Ed.; 53: 10242–10246 (2014).
3. Genetic Code Expansion Enables Live-Cell and Super-Resolution Imaging of Site-Specifically Labeled Cellular Proteins; C. Uttamapinant et al., J. Am. Chem. Soc.,137 (14), pp 4602–4605 (2015)
