COMING in 2020
In early 2020, we will introduce a whole suite of new fluorogenic live cell fluorescent probes working in the green, orange, red and far red channels:
Spirochrome’s SPYTM probes.
SPYTM probes are live cell, fluorogenic, photostable and STED/SIM compatible. They keep all the nice properties of our SiR-probes but work in different channels such as FITC or TMR. Probes for DNA (nucleus), actin and microtubules will be available.
Flipper-TR® the first fluorescent probe for mechanobiology now available from Spirochrome
Flipper-TR® is a live cell fluorescent membrane tension probe. It is the first fluorescent membrane tension reporter developed for the field of mechanobiology. The fluorescence lifetime of Flipper-TR® is strongly dependent on the membrane tension, therefore through FLIM imaging it is possible to measure precisely the spatio-temporal distribution of tension in membranes. Flipper-TR® opens up a whole new field by giving access to real time membrane tension measurements in live cells.
Discover our SiR probes
Spirochrome’s SiR probes are based on the fluorphore Silicon Rhodamine (SiR). Its key features are 1. cell permeability, 2. fluorogenic character and 3. compatibility with super-resolution microscopy. The fluorescence excitation and emission of SiR are in the far-red, reducing phototoxicity in live-cell imaging experiments. SiR is compatible with most microscopes as it can be used with standard Cy5 settings. The combination of all these properties set SiR-based probes apart from other fluorescent probes. Spirochrome SiR-probes work as simple as “add and image”
Spirochrome provides live cell imaging probes for DNA, Actin, Microtubules and lysosomes that are available in two colors: SiR and SiR700
SiR fluorophore and reagents
Spirochrome provides SiR-reagents for the preparation protein, nucleic acid, peptide or small molecules SiR-conjugates creating tailor-made probes. Currently, carboxylic acid, NHS ester, maleimide, alkyne, azide, tetrazine and BCN derivatives of SiR are available.
Alternatively, our chemists can provide you with custom SiR-derivatives and conjugates. Please contact us.