The FastAct™ live cell actin probes family expands with SPY555-FastAct™

We introduce SPY555-FastAct™ which expands our brand new FastAct™ product line into the orange part of the VIS spectrum.
Just like SPY650-FastAct™, SPY555-FastAct™ is a new and unique fluorescent live cell actin probe. It labels very dynamic actin filaments. SPY555-FastAct™ is a bright, fluorogenic, non toxic, cell permeable and highly specific live cell probe for fluorescence imaging of actin. It does not require any transfection and works just by adding the probe to the cells and perform imaging a couple of hours later.

Learn more about our new SPY555-FastAct™ probe here

Live COS7 cells labeled with SPY555-FastAct and Hoechst. Credit: Christophe Leterrier, INP CNRS-AMU UMR, Marseille, France

Discover Our SPY™ probes

Spirochrome’s SPY™ probes are revolutionary fluorogenic live cell fluorescent probes covering the whole visible spectrum

SPY™ probes are live cell, fluorogenic, photostable and STED/SIM compatible. They keep all the nice properties of our SiR-probes but work in different channels such as FITC, TMR or Texas Red. Probes for DNA (nucleus), actin and microtubules are available.

See our new SPY™ probes here

Flipper-TR® the first fluorescent probe for mechanobiology now available from Spirochrome

Flipper-TR® is a live cell fluorescent membrane tension probe. It is the first fluorescent membrane tension reporter developed for the field of mechanobiology. The fluorescence lifetime of Flipper-TR® is strongly dependent on the membrane tension, therefore through FLIM imaging it is possible to measure precisely the spatio-temporal distribution of tension in membranes. Flipper-TR® opens up a whole new field by giving access to real time membrane tension measurements in live cells.

Find out more about Flipper-TR® here

Discover our SiR probes

Spirochrome’s SiR probes are based on the fluorphore Silicon Rhodamine (SiR). Its key features are 1. cell permeability, 2. fluorogenic character and 3. compatibility with super-resolution microscopy. The fluorescence excitation and emission of SiR are in the far-red, reducing phototoxicity in live-cell imaging experiments. SiR is compatible with most microscopes as it can be used with standard Cy5 settings. The combination of all these properties set SiR-based probes apart from other fluorescent probes. Spirochrome SiR-probes work as simple as “add and image”

Spirochrome provides live cell imaging probes for DNA, Actin, Microtubules and lysosomes that are available in two colors: SiR and SiR700

Discover our products here

Drosophila hind gut stained with SiR-actin

SiR fluorophore and reagents

Spirochrome provides SiR-reagents for the preparation protein, nucleic acid, peptide or small molecules SiR-conjugates creating tailor-made probes. Currently, carboxylic acid, NHS ester, maleimide, alkyne, azide, tetrazine and BCN derivatives of SiR are available.

Discover SiR-reagents products here

Alternatively, our chemists can provide you with custom SiR-derivatives and conjugates. Please contact us.