To celebrate our 5th anniversary, we look very much forward to introducing a whole suite of new fluorogenic live cell fluorescent probes working in the green, orange, red and far red channels:

Spirochrome’s SPY-probes. Keep an eye on our website to SPY on us!

Flipper-TR® the first fluorescent probe for mechanobiology now available from Spirochrome

Flipper-TR® is a live cell fluorescent membrane tension probe. It is the first fluorescent membrane tension reporter developed for the field of mechanobiology. The fluorescence lifetime of Flipper-TR® is strongly dependent on the membrane tension, therefore through FLIM imaging it is possible to measure precisely the spatio-temporal distribution of tension in membranes. Flipper-TR® opens up a whole new field by giving access to real time membrane tension measurements in live cells.

Find out more about Flipper-TR® here

Discover our SiR probes

Spirochrome’s SiR probes are based on the fluorphore Silicon Rhodamine (SiR). Its key features are 1. cell permeability, 2. fluorogenic character and 3. compatibility with super-resolution microscopy. The fluorescence excitation and emission of SiR are in the far-red, reducing phototoxicity in live-cell imaging experiments. SiR is compatible with most microscopes as it can be used with standard Cy5 settings. The combination of all these properties set SiR-based probes apart from other fluorescent probes. Spirochrome SiR-probes work as simple as “add and image”

Spirochrome provides live cell imaging probes for DNA, Actin, Microtubules and lysosomes that are available in two colors: SiR and SiR700

Discover our products here

Drosophila hind gut stained with SiR-actin

SiR fluorophore and reagents

Spirochrome provides SiR-reagents for the preparation protein, nucleic acid, peptide or small molecules SiR-conjugates creating tailor-made probes. Currently, carboxylic acid, NHS ester, maleimide, alkyne, azide, tetrazine and BCN derivatives of SiR are available.

Discover SiR-reagents products here

Alternatively, our chemists can provide you with custom SiR-derivatives and conjugates. Please contact us.