Description
Flipper-TR is a fluorescent probe that specifically targets the plasma membrane of cells and reports membrane tension changes through its fluorescence lifetime changes. It is the most advanced member of the Flipper probes family2,3), which sense changes of the organization of lipid bilayer membranes through changes of the twist angle and polarization between the two twisted dithienothiophenes of the mechanophore. Flipper-TR spontaneously inserts into the plasma membrane of cells and is only fluorescent when inserted in a lipid membrane. It has a broad absorption and emission spectrum, excitation can be commonly performed with a 488nm laser, while emission is collected between 575 and 625nm. A detailed protocol for staining and FLIM imaging can be found in the datasheet. Flipper-TR works on a wide range of organisms including bacteria, yeast4), mammalians1) and plants. Probe quantity allows 50 – 200 staining experiments.*
Ordering:
You can request a quote by filling this form or place an order following these instructions:
We accept all types of purchase orders. If you want to place an order, please provide us with the following information and send it as pdf or email it to us at flipper@spirochrome.com:
- Quantity of SC020: number of 50 nmol Flipper-TR probe tubes
- Shipping Address, including name, phone and email of a contact person
- Billing Address, if different from Shipping Address
- Do you want to use your own Fedex account for shipping? If yes, please provide us with your Fedex account No
- Preferred Currency: USD, EUR or CHF
- Payment method: Wire transfer (preferred) or Credit Card (via PayPal). If you chose the latter, please provide the email address of the Card Holder. Our payment terms are 30 days, net, after receipt of the invoice.
Price without shipping:
Until 28-Feb-2019, the price for 1x SC020: 50 nmol Flipper-TR probe without shipping is 195 USD, 175 EUR or 195 CHF (shipping carried out via your Fedex account)
Shipping costs:
Shipping is carried out via Fedex Int’l Priority. Our price for that service is 45 USD, 40 EUR or 45 CHF.
Additional import costs from taxes, custom fees, etc. might occur at your expense
Orders are subject to our Terms and Conditions.
Photophysical properties
λabs 480 nm
λfl 600 nm
εmax 1.66·104 mol-1·cm-1 (in DMSO)
lifetime 2.8-7 ns
QY 30% (in AcOEt)
Downloads:
Flipper-TR Abs/Em spectra (Excel format)
References:
1) Colom A, et al: A fluorescent membrane tension probe. Nat Chem, 2018, 10:1118–1125 ().
2) Dal Molin M, et al: Fluorescent flippers for mechanosensitive membrane probes. JACS, 2015, 137:568-571.
3) Soleimanpour S, et al: Headgroup engineering in mechanosensitive membrane probes. Chem Commun (Camb) 2016, 52:14450-14453.
4) Riggi M, et al: Decrease in plasma membrane tension triggers PtdIns(4,5)P2 phase separation to inactivate TORC2, Nat Cell Biol, 2018 Sep; 20 (9): 1043–1051
*Based on the following conditions: 0.5 – 1 ml staining solution / staining experiments with 0.5 – 1 uM probe concentration. The number of staining experiments can be further increased by reducing volume or probe concentration.
Flipper-TR probe is distributed by Spirochrome under an exclusive license from the University of Geneva, Switzerland and was developed by the NCCR Chemical Biology.
