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Sir-DNAHuman fibroblasts stained with SiR-DNAMCF10A Spheroid stained with SiR-DNAexcitation and emission plot of SiR

SiR-DNA kit

CHF260.00

SiR-DNA (SiR-Hoechst*) is a far-red, fluorogenic, cell permeable and highly specific live cell DNA probe for fluorescence imaging of the nucleus and DNA.

Kit contains 50 nmol SiR-DNA and 1 umol verapamil

Price excludes VAT and shipping


Customers from North America (US and CA), please order from our dedicated supplier Cytoskeleton, Inc.

Customers from Europe (without Switzerland), please order from our dedicated supplier tebu-bio

cat#: SC007 Categories: Probes for Bioimaging, products, SiR probes
  • Description
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Product Description

SiR-DNA (SiR-Hoechst*) is a live cell nuclear stain based on the DNA minor groove binder bisbenzimide, it allows the labelling of DNA in live cells with high specificity and low background. SiR-DNA is fluorogenic, cell permeable and highly specific for DNA. Sir-DNA stains the nuclei of live cells without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SiR-DNA is compatible with GFP and/or m-cherry fluorescent proteins. SiR-DNA is a non cytotoxic alternative to DRAQ5. It can be imaged with standard Cy5 filtersets. SiR-DNA can be used for widefield, confocal, SIM or STED imaging in living cells and tissue. Probe quantity allows 50 – 200 staining experiments.**

Did you know? Our New product SPY650-DNA is an improved version of SiR-DNA. SPY650-DNA stains cells with brighter signal and with less background while still compatible with superresolution. Learn more about it here: SPY650-DNA

SPY650-DNA vs SiR-DNA

Optical properties

λabs 652 nm
εmax 1.0·105 mol-1·cm-1
λfl 674 nm

Reference: G. Lukinavicius et al.,SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy, Nat. Commun. 6:8497 doi: 10.1038/ncomms9497 (2015).

*SiR-DNA is identical to SiR-Hoechst, the name used for this probe in the above Nature Communications article.

**Based on the following conditions: 0.5 – 1 ml staining solution / staining experiments with 0.5 – 1 uM probe concentration. The number of staining experiments can be further increased by reducing volume or probe concentration.

SPY650-DNA kit datasheet

Verapamil usage guidelines

SPY650-DNA MSDS

Ex/Em spectra (excel format)

Related products

  • Cytoskeleton kit

    CHF670.00
    Show Details
  • SiR700-DNA kit

    CHF260.00
    Show Details
  • SiR700-tubulin kit

    CHF420.00
    Show Details
  • SiR-NHS ester

    From CHF320.00
    Select options
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