• 0Shopping Cart
Spirochrome
  • News
  • Products
    • Membrane tension probes
      • Flipper-TR®
      • ER Flipper-TR®
      • Mito Flipper-TR®
      • Lyso Flipper-TR®
    • SPY Probes
      • SPY505-DNA
      • SPY555-DNA
      • SPY555-tubulin
      • SPY555-FastAct™
      • SPY555-actin
      • SPY595-DNA
      • SPY620-actin
      • SPY620-DNA
      • SPY650-tubulin
      • SPY650-FastAct™
      • SPY650-DNA
      • SPY700-DNA
      • SPY700-FastAct™
    • PKmito probes
      • PKmito RED
      • PKmito ORANGE
      • PKmito DEEP RED
    • SiR Probes
      • SiR-DNA kit
      • SiR-actin kit
      • SiR-tubulin kit
      • SiR-lysosome kit
    • SiR700 Probes
      • SiR700-DNA kit
      • SiR700-actin kit
      • SiR700-tubulin kit
      • SiR700-lysosome kit
    • SiR Fluorophore & Reagents
      • SpyRho 555
      • SPY555-BG
      • SPY620-BG
      • SiR650-BG
      • SiR700-BG
      • SiR-COOH
      • SiR-NHS ester
      • SiR-Maleimide 1mg
      • SiR-azide 1mg
      • SiR-alkyne 1mg
      • SiR-tetrazine 1mg
      • SiR-BCN
  • About us
  • Technology
  • References
  • Gallery
  • FAQ
  • Contact
  • Menu Menu
  • Mastodon
  • Twitter
Sir-DNAHuman fibroblasts stained with SiR-DNAMCF10A Spheroid stained with SiR-DNAexcitation and emission plot of SiR

SiR-DNA kit

SiR-DNA (SiR-Hoechst*) is a far-red, fluorogenic, cell permeable and highly specific live cell DNA probe for fluorescence imaging of the nucleus and DNA.

Kit contains 50 nmol SiR-DNA and 1 umol verapamil

cat#: SC007 Categories: Probes for Bioimaging, products, SiR probes
  • Description
  • Downloads

Product Description

SiR-DNA (SiR-Hoechst*) is a live cell nuclear stain based on the DNA minor groove binder bisbenzimide, it allows the labelling of DNA in live cells with high specificity and low background. SiR-DNA is fluorogenic, cell permeable and highly specific for DNA. Sir-DNA stains the nuclei of live cells without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SiR-DNA is compatible with GFP and/or m-cherry fluorescent proteins. SiR-DNA is a non cytotoxic alternative to DRAQ5. It can be imaged with standard Cy5 filtersets. SiR-DNA can be used for widefield, confocal, SIM or STED imaging in living cells and tissue. Probe quantity allows 50 – 200 staining experiments.**

Did you know? Our New product SPY650-DNA is an improved version of SiR-DNA. SPY650-DNA stains cells with brighter signal and with less background while still compatible with superresolution. Learn more about it here: SPY650-DNA

SPY650-DNA vs SiR-DNA

Optical properties

λabs 652 nm
εmax 1.0·105 mol-1·cm-1
λfl 674 nm

Reference: G. Lukinavicius et al.,SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy, Nat. Commun. 6:8497 doi: 10.1038/ncomms9497 (2015).

*SiR-DNA is identical to SiR-Hoechst, the name used for this probe in the above Nature Communications article.

**Based on the following conditions: 0.5 – 1 ml staining solution / staining experiments with 0.5 – 1 uM probe concentration. The number of staining experiments can be further increased by reducing volume or probe concentration.

SiR-DNA kit datasheet
Verapamil usage guidelines
SiR-DNA SDS
Ex/Em spectra (excel format)

Related products

  • SiR-BCN

    Show Details
  • SiR-tetrazine 1mg

    Show Details
  • SiR-Maleimide 1mg

    Show Details
  • SiR-azide 1mg

    Show Details
© Copyright - Spirochrome - Terms and conditions - privacy policy
SiR-tetrazine 1mgSiR-azide 1mg
Scroll to top

This site uses cookies and reCAPTCHA v3. By clicking the "OK" button and continuing to browse the site, you are agreeing to our use of cookies and reCAPTCHA v3. For more information see our privacy policy

OK


How we use cookies

We may request cookies to be set on your device. We use cookies to let us know when you visit our websites, how you interact with us, to enrich your user experience, and to customize your relationship with our website.

Click on the different category headings to find out more. You can also change some of your preferences. Note that blocking some types of cookies may impact your experience on our websites and the services we are able to offer.

Essential Website Cookies

These cookies are strictly necessary to provide you with services available through our website and to use some of its features.

Because these cookies are strictly necessary to deliver the website, refusing them will have impact how our site functions. You always can block or delete cookies by changing your browser settings and force blocking all cookies on this website. But this will always prompt you to accept/refuse cookies when revisiting our site.

We fully respect if you want to refuse cookies but to avoid asking you again and again kindly allow us to store a cookie for that. You are free to opt out any time or opt in for other cookies to get a better experience. If you refuse cookies we will remove all set cookies in our domain.

We provide you with a list of stored cookies on your computer in our domain so you can check what we stored. Due to security reasons we are not able to show or modify cookies from other domains. You can check these in your browser security settings.

Google Analytics Cookies

These cookies collect information that is used either in aggregate form to help us understand how our website is being used or how effective our marketing campaigns are, or to help us customize our website and application for you in order to enhance your experience.

If you do not want that we track your visit to our site you can disable tracking in your browser here:

Other external services

We also use different external services like Google Webfonts, Google Maps, and external Video providers. Since these providers may collect personal data like your IP address we allow you to block them here. Please be aware that this might heavily reduce the functionality and appearance of our site. Changes will take effect once you reload the page.

Google Webfont Settings:

Google Map Settings:

Google reCaptcha Settings:

Vimeo and Youtube video embeds:

Other cookies

The following cookies are also needed - You can choose if you want to allow them:

Privacy Policy

You can read about our cookies and privacy settings in detail on our Privacy Policy Page.

privacy_policy
Accept settingsHide notification only