Sir-DNAHuman fibroblasts stained with SiR-DNAMCF10A Spheroid stained with SiR-DNAexcitation and emission plot of SiR

SiR-DNA kit

CHF280.00

SiR-DNA (SiR-Hoechst*) is a far-red, fluorogenic, cell permeable and highly specific probe for DNA

Kit contains 50 nmol SiR-DNA and 1 umol verapamil

Customers from North America (US and CA), please order from our dedicated supplier Cytoskeleton, Inc.

Customers from Europe (without Switzerland), please order from our dedicated supplier tebu-bio

cat#: SC007 Categories: , ,

Description

SiR-DNA (SiR-Hoechst*) is based on the DNA minor groove binder bisbenzimide, it allows the labelling of DNA in live cells with high specificity and low background. SiR-DNA is fluorogenic, cell permeable and highly specific for DNA. Sir-DNA stains the nuclei of live cells without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SiR-DNA is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with standard Cy5 filtersets. SiR-DNA can be used for widefield, confocal, SIM or STED imaging in living cells and tissue. Probe quantity allows 50 – 200 staining experiments.**

Optical properties

λabs 652 nm
εmax 1.0·105 mol-1·cm-1
λfl 674 nm

Reference: G. Lukinavicius et al.,SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy, Nat. Commun. 6:8497 doi: 10.1038/ncomms9497 (2015).

*SiR-DNA is identical to SiR-Hoechst, the name used for this probe in the above Nature Communications article.

**Based on the following conditions: 0.5 – 1 ml staining solution / staining experiments with 0.5 – 1 uM probe concentration. The number of staining experiments can be further increased by reducing volume or probe concentration.

SiR-DNA datasheet

Verapamil usage guidelines

SiR-DNA MSDS

Ex/Em spectra (excel format)

1. How many experiments can I do with 50 nmol of SiR-DNA?
Depending on the sample volume and the concentration used for labelling, about 50-200 experiments can be done with one vial. 
2. Is SiR-DNA stable at room temperature?
Yes, the probe is stable at room temperature for a few days. However, it is recommended to store SiR-DNA or solutions thereof at -20°C to maximize shelf-life. 
3. Does SiR-DNA stain fixed cells?
Yes, SiR-DNA can be used with methanol or PFA fixed cells. However, the mounting medium has a strong influence on the brightness of SiR-DNA. While cells mounted with PBS or Vectashield® gave a low signal, cells mounted with pure glycerol had very bright nuclei. We therefore recommend to use pure glycerol as mounting medium when staining fixed cells with SiR-DNA.
4. Is SiR-DNA toxic to the cells?
SiR-DNA did not impair cell proliferation up to 25 uM. In addidion, it did not prolong mitotic progression nor increase the incidence of lagging or bridged anaphase chromosomes at concentrations up to 10 uM. Therefore SiR-DNA is safe to use on cells even for prolonged time laps imaging. 
5. Which organisms and tissues are stained by Sir-DNA?
This list is only reporting known working cell lines, tissues or organisms. If a cell line, tissue or organism is not present in the list, it does not mean that it will not be stained by SiR-Probes.

Homo sapiens : U2OS, fibroblasts, HeLa MCF-10A, A549, erythrocytes.

Drosophila melanogaster : Notum epithelium, S2.

Help us to complete the list by reporting your positive experiences! 

6. Do SiR-probes work in 3D cell cultures?
Yes, the probes are able to stain cells in a 3D growth environment.
7. What are the correction factors CF260 and CF280 for SiR fluorophore?

CF260 = 0.116

CF280 = 0.147

8. Why are your prices displayed only in CHF (Swiss Francs)?
Our company is based in Switzerland and therefore our list prices are displayed in CHF (Swiss Francs). Upon request, we will provide you with a binding quote (valid 1 month) based on the displayed CHF prices and the actual exchange rate. Please indicate your preferred currency (USD, EUR, CHF) and payment method (bank transfer OR credit card via PayPal).
9. Where can I order my Spirochrome products?
Spirochrome has appointed dedicated distributors for the SiR-probes: SiR-actin, SiR-tubulin, & cytoskeleton-kit and SiR-DNA. Customers from North America (US and CA) can order them through Cytoskeleton, Inc. and European customers (without Switzerland) can order them through tebu-bio.
The reagents: SiR-COOH, SiR-NHS, & Boc-Lys-jasplakinolide, can be ordered through our website via email or the webshop.

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