Description
SiR-DNA (SiR-Hoechst*) is a live cell nuclear stain based on the DNA minor groove binder bisbenzimide, it allows the labelling of DNA in live cells with high specificity and low background. SiR-DNA is fluorogenic, cell permeable and highly specific for DNA. Sir-DNA stains the nuclei of live cells without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SiR-DNA is compatible with GFP and/or m-cherry fluorescent proteins. SiR-DNA is a non cytotoxic alternative to DRAQ5. It can be imaged with standard Cy5 filtersets. SiR-DNA can be used for widefield, confocal, SIM or STED imaging in living cells and tissue. Probe quantity allows 50 – 200 staining experiments.**
Optical properties
λabs 652 nm
εmax 1.0·105 mol-1·cm-1
λfl 674 nm
Reference: G. Lukinavicius et al.,SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy, Nat. Commun. 6:8497 doi: 10.1038/ncomms9497 (2015).
*SiR-DNA is identical to SiR-Hoechst, the name used for this probe in the above Nature Communications article.
**Based on the following conditions: 0.5 – 1 ml staining solution / staining experiments with 0.5 – 1 uM probe concentration. The number of staining experiments can be further increased by reducing volume or probe concentration.
