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SPY555-BG
SPY555-BG

SPY555-BG

SPY555-BG is a bright, cell permeable and orange emitting benzylguanine substrate for labeling SNAP-tag™* in cellular fluorescence imaging experiments.

Product contains 1 vial of SPY555-BG  (35 nmol) in lyophilised form allowing to prepare 35 ul of 400x DMSO stock solution.


SKU: SC204 Category: Fluorophore & Reagents
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Product Description

SPY555-BG is the benzylguanine derivative of our SPY555 fluorophore. It emits light in the orange part of the UV-ViS spectrum, it is fluorogenic, highly cell permeable and well suited for STED and SIM superresolution imaging. SPY555-BG can be imaged with a standard Cy3 filterset. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. It has the same structure as MaP555-SNAP the name used in its original publication in Nature Chemistry1). SPY555-BG is an alternative to SNAP-Cell® TMR-Star, it has faster labeling kinetics.

Benzylguanine (BG) is the substrate of the self labeling tag SNAP-tag™*. Upon reaction with a BG derivative, SNAP-tag™* forms a covalent bond with the substrate and releases guanine. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as SNAP-tag™* fusion.

Probe Properties

 

Absorbance maximum λabs 555 nm
Fluorescence maximum λfl 580 nm
Works on fixed cells? Yes, any type of fixation method
Probe quantity 35 nmol
Fluorescence lifetime 2.4 ns
STED depletion wavelength 660 or 775 nm
Shipping room temperature
Storage -20°C

 

1. “A general strategy to develop cell permeable and fluorogenic probes for multicolour nanoscopy”; Lu Wang, et. al.; Nature Chemistry 12, 165-172, 2020.

*SNAP-tag™ and SNAP-Cell® are registered trademarks of New England Biolabs, Inc.

Latest citing articles

SPY555-BG datasheet

SPY555-BG SDS

Ex/Em spectra (excel format)

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