Product Description
SPY555-BG is the benzylguanine derivative of our SPY555 fluorophore. It emits light in the orange part of the UV-ViS spectrum, it is fluorogenic, highly cell permeable and well suited for STED and SIM superresolution imaging. SPY555-BG can be imaged with a standard Cy5 filterset. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. It has the same structure as MaP555-SNAP the name used in its original publication in Nature Chemistry1). SPY555-BG is an alternative to SNAP-Cell® TMR-Star, it has faster labeling kinetics.
Benzylguanine (BG) is the substrate of the self labeling tag SNAP-tag™*. Upon reaction with a BG derivative, SNAP-tag™* forms a covalent bond with the substrate and releases guanine. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as SNAP-tag™* fusion.
Probe Properties
| Absorbance maximum λabs | 555 nm |
| Fluorescence maximum λfl | 580 nm |
| Works on fixed cells? | Yes, any type of fixation method |
| Probe quantity | 35 nmol |
| Fluorescence lifetime | 2.4 ns |
| STED depletion wavelength | 660 or 775 nm |
| Shipping | room temperature |
| Storage | -20°C |
1. “A general strategy to develop cell permeable and fluorogenic probes for multicolour nanoscopy”; Lu Wang, et. al.; Nature Chemistry 12, 165-172, 2020.
*SNAP-tag™ and SNAP-Cell® are registered trademarks of New England Biolabs, Inc.
