Product Description
SiR-alkyne is the N-propargylcarboxamide derivative of the fluorophore silicon rhodamine (SiR). Sir-alkyne reacts with azide derivatives in the presence of copper (I) to form 1,2,3-triazoles linkage. Small molecules, peptides, oligonucleotides or proteins bearing the appropriate azide can be conjugated to SiR-alkyne. SiR excitation and emission are compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with a standard Cy5 filterset.
Properties
λabs 652 nm
εmax 1.0·105 mol-1·cm-1
λfl 674 nm
MW =509.7 g/mol
CF260 = 0.116
CF280 = 0.147
References
1. A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins; G. Lukinavičius et al.; Nature Chemistry; 5, 132–139 (2013).
