Product Description
PKmito Orange FX (also known as PKMO FX) is a bright, photostable, mitochondrial probe based on the PKmito™ dyes developed in the lab of Zhixing Chen at Peking University1). PKmito Orange FX labels mitochondria in formaldehyde or glutaraldehyde fixed cells with very high specificity. The unique and unmatched feature of PKmito Orange FX is its ability to be retained nearly quantitatively after glutaraldehyde fixation of stained cells. PKmito Orange FX is extremely well suited for STED and SIM superresolution imaging. It allows to perform nanoscopy of mitochondria with exquisite resolution and bright signal. PKmito Orange FX accumulates in the mitochondrial inner membrane (IM) and stays in place upon fixation. It is highly suited to image mitochonridal cristae structure by STED superresolution microscopy using a 775 nm depletion line. PKmito Orange FX does not require any genetic manipulation, transfection or overexpression of fluorescent proteins. PKmito Orange FX enables multicolor imaging with SPY505, SPY650, SPY700, SiR or GFP. It can be used for widefield, confocal, SIM or STED imaging in glutaraldehyde fixed cells and tissue. Contains 1 vial of PKmito Orange FX (lyophilized).
Probe Properties
| Absorbance maximum λabs | 584 nm |
| Fluorescence maximum λfl | 604 nm |
| Works on fixed cells? | Yes, FA and GA fixes cells |
| Probe quantity | 100 stainings* |
| Fluorescence lifetime | n.d. |
| STED depletion wavelength | 775 nm |
| Shipping | room temperature |
| Storage | -20°C |
*Based on the following conditions: 0.5 ml staining solution / staining experiment with 1x probe concentration. The number of staining experiments can be further increased by reducing the staining solution volume or the probe concentration.
1. Jingting Chen et Al. “An Aldehyde-crosslinking Mitochondrial Probe for Nanoscopic Imaging in Fixed Cells”, PNAS (2024)
