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PKmito ORANGE

PKmito ORANGE: Probe for live cell mitochondria imaging
PKmito ORANGE (also known as PKMO) is a unique fluorescent probe for live cell mitochondria imaging. Due to its very low phototoxicity, high photostability and high brightness, it is highly suited to image mitochonridal cristae structure and dynamics by STED superresolution microscopy using a 775 nm depletion line. PKmito ORANGE does not require any transfection and works just by adding the probe to the cells and image 30 minutes later.

Product contains 1 vial of PKmito ORANGE in lyophilised form allowing to prepare 50 ul of 1000x DMSO stock solution (100 stainings*).

cat#: SC053 Category: PKmito probes
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Product Description

PKmito Orange (also known as PKMO) is a bright, photostable, non-phototoxic mitochondrial probe based on the PKmito™ dyes developed in the lab of Zhixing Chen at Peking University1,2). PKmito Orange labels mitochondria in live cells with very high specificity. The unique and unmatched feature of PKmito Orange is its extremely low phototoxicity, due to the presence of the intramolecular triplet quencher cyclooctatetraene (COT) group. It allows to perform long term imaging of mitochondria without damaging them. PKmito Orange accumulates in the mitochondrial inner membrane (IM). It is highly suited to image mitochonridal cristae structure and dynamics by STED superresolution microscopy using a 775 nm depletion line. PKmito Orange does not require any genetic manipulation, transfection or overexpression of fluorescent proteins. PKmito Orange enables multicolor imaging with SPY505, SPY650, SPY700, SiR or GFP. It can be used for widefield, confocal, SIM or STED imaging in living cells and tissue. Contains 1 vial of PKmito Orange (lyophilized).

 

Probe Properties

 

Absorbance maximum λabs 591 nm
Fluorescence maximum λfl 608 nm
Works on fixed cells? No
Probe quantity 100 stainings*
Fluorescence lifetime 1.8 ns
STED depletion wavelength 775 nm
Shipping room temperature
Storage -20°C


*Based on the following conditions: 0.5 ml staining solution / staining experiment with 1x probe concentration. The number of staining experiments can be further increased by reducing the staining solution volume or the probe concentration.

1. Liu Tianyan et al. “Multi-color live-cell STED nanoscopy of mitochondria with a gentle inner membrane stain” PNAS. 2022; 119(52):e2215799119

2. Yang, Zhongtian, et al. “Cyclooctatetraene-conjugated cyanine mitochondrial probes minimize phototoxicity in fluorescence and nanoscopic imaging.” Chemical science 11.32 (2020): 8506-8516.

 

PKmito ORANGE datasheet

PKmito ORANGE SDS

Ex/Em spectra (excel format)

Related products

  • PKmito RED

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  • PKmito DEEP RED

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