PKmito Orange (also known as PKMO) is a bright, photostable, non-phototoxic mitochondrial probe based on the PKmito™ dyes developed in the lab of Zhixing Chen at Peking University1,2). PKmito Orange labels mitochondria in live cells with very high specificity. The unique and unmatched feature of PKmito Orange is its extremely low phototoxicity, due to the presence of the intramolecular triplet quencher cyclooctatetraene (COT) group. It allows to perform long term imaging of mitochondria without damaging them. PKmito Orange accumulates in the mitochondrial inner membrane (IM). It is highly suited to image mitochonridal cristae structure and dynamics by STED superresolution microscopy using a 775 nm depletion line. PKmito Orange does not require any genetic manipulation, transfection or overexpression of fluorescent proteins. PKmito Orange enables multicolor imaging with SPY505, SPY650, SPY700, SiR or GFP. It can be used for widefield, confocal, SIM or STED imaging in living cells and tissue. Contains 1 vial of PKmito Orange (lyophilized).
|Absorbance maximum λabs||591 nm|
|Fluorescence maximum λfl||608 nm|
|Works on fixed cells?||No|
|Probe quantity||100 stainings*|
|Fluorescence lifetime||1.8 ns|
|STED depletion wavelength||775 nm|
*Based on the following conditions: 0.5 ml staining solution / staining experiment with 1x probe concentration. The number of staining experiments can be further increased by reducing the staining solution volume or the probe concentration.
1. Liu Tianyan et al. “Multi-color live-cell STED nanoscopy of mitochondria with a gentle inner membrane stain” PNAS. 2022; 119(52):e2215799119
2. Yang, Zhongtian, et al. “Cyclooctatetraene-conjugated cyanine mitochondrial probes minimize phototoxicity in fluorescence and nanoscopic imaging.” Chemical science 11.32 (2020): 8506-8516.