SiR700-lysosome kit

CHF350.00

SiR700-lysosome is a fluorogenic, cell permeable and highly specific probe for DNA

Kit contains 35 nmol SiR700-lysosome and 1 umol verapamil

Customers from North America (US and CA), please order from our dedicated supplier Cytoskeleton, Inc.

Customers from Europe (without Switzerland), please order from our dedicated supplier tebu-bio

cat#: SC016 Categories: , ,

Description

SiR700-lysosome is based on the cathepsin D binding natural product pepstatin A. SiR700-lysosome is fluorogenic, cell permeable and highly specific for lysosomes. Sir700-lysosome stains lysosomes in ilve cells without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SiR700-lysosome is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with standard Cy5 filtersets. SiR-actin can be used for widefield, confocal, SIM or STED imaging in living cells and tissue. Probe quantity allows 50 – 200 staining experiments.*

In combination with standard SiR-probes, SiR700-lysosome allows dual colour imaging experiments in live cells. For more information please visit www.spirochrome.com/dualcolour.

Optical properties

λabs 682 nm
εmax 1.0·105 mol-1·cm-1
λfl 716 nm
Recommended STED depletion line: 810 nm

 

 

*Based on the following conditions: 0.5 – 1 ml staining solution / staining experiments with 0.5 – 1 uM probe concentration. The number of staining experiments can be further increased by reducing volume or probe concentration.

SiR700-lysosome kit datasheet

Verapamil usage guidelines

SiR700-lysosome MSDS

Ex/Em spectra (excel format)

1. How many experiments can I do with 50 nmol of SiR-actin?
Depending on the sample volume and the concentration used for labelling, about 50-200 experiments can be done with one vial. 
2. Is SiR-actin stable at room temperature?
Yes, the probe is stable at room temperature for a few days. However, it is recommended to store SiR-actin or solutions thereof at -20°C to maximize shelf-life. 
3. Does SiR-actin stain fixed cells?
Yes, SiR-actin can be used with PFA fixed cells. SiR-actin labels F-actin in PFA fixed cells as efficiently as Phalloidin-derivatives using identical protocol. Sir-actin is not suitable for methanol fixed cells. 
4. Is SiR-actin toxic to the cells?
Yes, above a certain threshold SiR-actin shows some effect on cell proliferation and altered actin dynamics. However, the probe is orders of magnitude less toxic that the parent drug jasplakinolide. In HeLa cells actin dynamics were not altered at concentration below 100 nM. At this concentration, SiR-actin efficiently labels F-actin allowing to record high signal to noise images. 
5. Which organisms and tissues are stained by Sir-actin?
This list is only reporting known working cell lines, tissues or organisms. If a cell line, tissue or organism is not present in the list, it does not mean that it will not be stained by SiR-Probes.

Homo sapiens : U2OS, fibroblasts, HeLa, HUVEC, MCF-10A, HCT-116, A549, erythrocytes.

Mus musculus : C2C12, IA32, skeletal muscle, primary cardiomyocyte, primary oocyte.

Rattus norvegicus : primary hyppocampal neurons, primary cortex neurons NRK.

Cercopithecus aethiops : COS-7.

Mesocricetus auratus : BHK.

Drosophila melanogaster : Notum epithelium, S2.

Didelphis marsupialis : OK-cells.

Help us to complete the list by reporting your positive experiences! 

6. Do SiR-probes work in 3D cell cultures?
Yes, the probes are able to stain cells in a 3D growth environment.
7. What are the correction factors CF260 and CF280 for SiR fluorophore?

CF260 = 0.116

CF280 = 0.147

8. Why are your prices displayed only in CHF (Swiss Francs)?
Our company is based in Switzerland and therefore our list prices are displayed in CHF (Swiss Francs). Upon request, we will provide you with a binding quote (valid 1 month) based on the displayed CHF prices and the actual exchange rate. Please indicate your preferred currency (USD, EUR, CHF) and payment method (bank transfer OR credit card via PayPal).
9. Where can I order my Spirochrome products?
Spirochrome has appointed dedicated distributors for the SiR-probes: SiR-actin, SiR-tubulin, & cytoskeleton-kit. Customers from North America (US and CA) can order them through Cytoskeleton, Inc. and European customers (without Switzerland) can order them through tebu-bio.
The reagents: SiR-COOH, SiR-NHS, & Boc-Lys-jasplakinolide, can be ordered through our website via email or the webshop.

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