SiR700-lysosome is based on the cathepsin D binding natural product pepstatin A. SiR700-lysosome is fluorogenic, cell permeable and highly specific for lysosomes. Sir700-lysosome stains lysosomes in ilve cells without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SiR700-lysosome is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with standard Cy5 filtersets. SiR-actin can be used for widefield, confocal, SIM or STED imaging in living cells and tissue. Probe quantity allows 50 – 200 staining experiments.*
In combination with standard SiR-probes, SiR700-lysosome allows dual colour imaging experiments in live cells. For more information please visit www.spirochrome.com/dualcolour.
λabs 682 nm
εmax 1.0·105 mol-1·cm-1
λfl 716 nm
Recommended STED depletion line: 810 nm
*Based on the following conditions: 0.5 – 1 ml staining solution / staining experiments with 0.5 – 1 uM probe concentration. The number of staining experiments can be further increased by reducing volume or probe concentration.