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SiR620 Absorption and Emission Spectra

SPY620-actin

CHF415.00

SPY620-actin is a bright fluorogenic, non toxic, cell permeable and highly specific live cell probe for fluorescence imaging of actin.

Product contains 1 vial of SPY620-actin in lyophilised form allowing to prepare 50 ul of 1000x DMSO stock solution (100 stainings*).

Price excludes VAT and shipping


Customers from North America (US and CA), please order from our dedicated supplier Cytoskeleton, Inc.

Customers from Europe (without Switzerland), please order from our dedicated supplier tebu-bio

cat#: SC402 Category: SPY probes
  • Description
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Product Description

SPY620-actin is a bright red & non toxic live and fixed cells F-actin stain based on our SPY™ dyes series. Its optimized structure allows quick labeling of F-actin in live & fixed cells with high specificity and very low background. SPY620-actin is identical to MAP618-actin described in the nature chemistry article by the lab of Kai Johnsson. SPY620-actin stains F-actin of live or fixed cells without the need for genetic manipulation or overexpression of fluorescent proteins. Its emission in the red minimizes phototoxicity and sample autofluorescence. SPY620-actin enables multicolor imaging with SPY505, SPY555, SPY650, SPY700, SiR & GFP. SPY620-actin can be imaged with e.g. using 610/20 nm and 650/40 nm excitation and emission filters respectively. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. Probe quantity allows 100 staining experiments.*

Probe Properties

 

Absorbance maximum λabs 619 nm
Fluorescence maximum λfl 636 nm
Works on fixed cells? Yes, PFA fixed cells
Probe quantity 100 stainings*
Fluorescence lifetime 2.9 ns
STED depletion wavelength 775 nm
Shipping room temperature
Storage -20°C

 

*Based on the following conditions: 0.5 ml staining solution / staining experiment with 1x probe concentration. The number of staining experiments can be further increased by reducing the staining solution volume or the probe concentration.

SPY620-actin datasheet

SPY620-actin MSDS

Ex/Em spectra (excel format)

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  • SPY700-DNA

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