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Media Credits:

Christophe Leterrier, INP CNRS-AMU UMR, Marseille, France for the movie of COS7 cells labeled with SPY555-FastAct + Hoechst as well as the neuronal axon actin rings labeled with SPY555-FastAct

SPY555-FastAct™

F-actin imaging 2.0 with SPY555-FastAct™
SPY555-FastAct™ is a new and unique fluorescent live cell actin probe. It labels very dynamic actin filaments as well as the more static ones. SPY555-FastAct™ is a bright, fluorogenic, non toxic, cell permeable and highly specific live cell probe for fluorescence imaging of actin. It does not require any transfection and works just by adding the probe to the cells and perform imaging a couple of hours later.

Product contains 1 vial of SPY555-FastAct™ in lyophilised form allowing to prepare 50 ul of 1000x DMSO stock solution (100 stainings*).

cat#: SC205 Categories: Cytoskeleton probes, SPY probes, SPY555 probes
  • Description
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Product Description

SPY555-FastAct™ is a bright, orange, fluorogenic & non toxic F-actin stain based on our SPY™ dyes series. Its optimized structure allows to label F-actin in live cells with high specificity and low background. The unique and unmatched feature of SPY555-FastAct™ is its ability to label very dynamic actin, like LifeAct™** but the probe does not require any genetic manipulation, transfection or overexpression of fluorescent proteins. SPY555-FastAct™ is based on our bright, photostable & orange SPY555 fluorophore which is far superior than fluorescent proteins.

SPY555-FastAct™ enables multicolor imaging with SPY505, SPY595, SPY650, SPY700, SiR and GFP. SPY555-FastAct™ can be imaged with standard TMR or Cy3 settings. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. Probe quantity allows 100 staining experiments.*

 

Probe Properties

 

Absorbance maximum λabs 555 nm
Fluorescence maximum λfl 580 nm
Works on fixed cells? Yes, PFA fixed cells
Probe quantity 100 stainings*
Fluorescence lifetime 2.4 ns
STED depletion wavelength 660 nm or 775 nm
Shipping room temperature
Storage -20°C

 

*Based on the following conditions: 0.5 ml staining solution / staining experiment with 1x probe concentration. The number of staining experiments can be further increased by reducing the staining solution volume or the probe concentration.

**LifeAct™ is a registered trademark of Ibidi GmbH.

SPY555-FastAct datasheet

SPY555-FastAct SDS

Ex/Em spectra (excel format)

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