SPY555-FastAct™ is a bright, orange, fluorogenic & non toxic F-actin stain based on our SPY™ dyes series. Its optimized structure allows to label F-actin in live cells with high specificity and low background. The unique and unmatched feature of SPY555-FastAct™ is its ability to label very dynamic actin, like LifeAct™** but the probe does not require any genetic manipulation, transfection or overexpression of fluorescent proteins. SPY555-FastAct™ is based on our bright, photostable & orange SPY555 fluorophore which is far superior than fluorescent proteins.
SPY555-FastAct™ enables multicolor imaging with SPY505, SPY595, SPY650, SPY700, SiR and GFP. SPY555-FastAct™ can be imaged with standard TMR or Cy3 settings. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. Probe quantity allows 100 staining experiments.*
|Absorbance maximum λabs||555 nm|
|Fluorescence maximum λfl||580 nm|
|Works on fixed cells?||Yes, PFA fixed cells|
|Probe quantity||100 stainings*|
|Fluorescence lifetime||2.4 ns|
|STED depletion wavelength||660 nm or 775 nm|
*Based on the following conditions: 0.5 ml staining solution / staining experiment with 1x probe concentration. The number of staining experiments can be further increased by reducing the staining solution volume or the probe concentration.
**LifeAct™ is a registered trademark of Ibidi GmbH.