Description
SiR-azide is the 3-azidopropyl derivative of the fluorophore silicon rhodamine (SiR). Sir-azide reacts with terminal alkyne derivatives in the presence of copper (I) to form 1,2,3-triazoles linkage. It can also react with strained alkynes such as DIFO or DIBO without the need of copper. Small molecules, peptides, oligonucleotides or proteins bearing the appropriate alkyne can be conjugated to SiR-azide. . SiR is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with a standard Cy5 filterset.
Properties
λabs 652 nm
εmax 1.0·105 mol-1·cm-1
λfl 674 nm
MW =554.7 g/mol
CF260 = 0.116
CF280 = 0.147
References
1. A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins; G. Lukinavičius et al.; Nature Chemistry; 5, 132–139 (2013).
