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Sir-C3-azideexcitation and emission plot of SiR

SiR-azide 1mg

SiR-azide is a clickable derivative of SiR. It reacts with alkynes and allows the preparation of custom SiR-conjugates

Quantity: 1mg

cat#: SC009 Categories: Fluorophore & Reagents, products
  • Description
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Product Description

SiR-azide is the 3-azidopropyl derivative of the fluorophore silicon rhodamine (SiR). Sir-azide reacts with terminal alkyne derivatives in the presence of copper (I) to form 1,2,3-triazoles linkage. It can also react with strained alkynes such as DIFO or DIBO without the need of copper. Small molecules, peptides, oligonucleotides or proteins bearing the appropriate alkyne can be conjugated to SiR-azide. . SiR is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with a standard Cy5 filterset.

Properties

λabs 652 nm
εmax 1.0·105 mol-1·cm-1
λfl 674 nm
MW =554.7 g/mol
CF260 = 0.116
CF280 = 0.147

References

1. A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins; G. Lukinavičius et al.; Nature Chemistry; 5, 132–139 (2013).

SiR-azide datasheet

SiR-azide SDS

Ex/Em spectra (excel format)

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