We launch SPyRho 555, a new fluorogenic dye for live cell RNA imaging.
SpyRho 555 is a fluorogenic dye ligand for the RNA aptamer RhoBAST developed by the lab of Murat Sunbul in Heidelberg University1). SpyRho 555 is a rhodamine dye that is cell permeable, highly fluorogenic and photostable. With its high affinity and fast on/off binding kinetics to its cognate aptamer RhoBAST, it allows overcoming the main issues of live cell RNA imaging. SpyRho 555 is highly suited for widefield, confocal, STED or single molecule localization microscopy in a PAINT like setup.
Figure 1. SpyRho 555 reversibly binds to RhoBAST aptamer with fast kinetics. Unbound SpyRho 555 exists predominantly in the closed form and is not fluorescent. Upon binding to RhoBAST aptamer, SpyRho 555 switches to the open form and becomes highly fluorescent.
1. Daniel Englert et al. “Fast-exchanging spirocyclic rhodamine probes for aptamer-based super-resolution RNA imaging” bioRxiv 2022.10.24.513449
