Product Description
Take advantage of 3 ChloroAlkane (CA) Halotag™* substrates in a single Kit. All Halotag™* substrates are fluorogenic, highly cell permeable and well suited for widefiled, confocal, STED and SIM superresolution imaging.
SPY505-CA can be imaged in a “green” channel (e.g. FITC or GFP)
SPY555-CA can be imaged in an “orange” channel (e.g. TMR or Cy3)
SiR-CA can be imaged in a “far red” channel (e.g. Cy5)
ChloroAlkane (CA) is the substrate of the self labeling tag Halo-tag™*. Upon reaction with a CA derivative, Halo-tag™* forms a covalent bond with the substrate. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as Halo-tag™* fusion.
Probes Properties
| SPY505-CA | SPY555-CA | SiR | |
| Absorbance maximum λabs | 512 nm | 555 nm | 652 nm |
| Fluorescence maximum λfl | 532 nm | 580 nm | 674 nm |
| Works on fixed cells? | yes | ||
| Probe quantity | 3 nmol | 3 nmol | 3 nmol |
| Fluorescence lifetime | 4.0 ns | 2.3 ns | 3.2 ns |
| STED depletion wavelength | 595 mn | 660 or 775 nm | 775 nm |
| Shipping | room temperature | ||
| Storage | -20°C |
*Halo-tag™ is a registered trademark of Promega Corporation.
