Product Description
SPY505-CA is the ChloroAlkane (CA) derivative of our SPY505 fluorophore. It emits light in the green part of the UV-ViS spectrum, it is fluorogenic, highly cell permeable and well suited for STED and SIM superresolution imaging. SPY505-CA can be imaged with standard FITC or YFP settings. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. It has the same structure as MaP510-halo, the name used in its original publication in Nature Chemistry1).
ChloroAlkane (CA) is the substrate of the self labeling tag Halo-tag™*. Upon reaction with a CA derivative, Halo-tag™* forms a covalent bond with the substrate. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as Halo-tag™* fusion.
Probe Properties
| Absorbance maximum λabs | 512 nm |
| Fluorescence maximum λfl | 531 nm |
| Works on fixed cells? | Yes, any type of fixation method |
| Probe quantity | 10 nmol |
| Fluorescence lifetime | 4.0 ns |
| STED depletion wavelength | 595 nm |
| Shipping | room temperature |
| Storage | -20°C |
1. “A general strategy to develop cell permeable and fluorogenic probes for multicolour nanoscopy”; Lu Wang, et. al.; Nature Chemistry 12, 165-172, 2020.
**Based on the following conditions: 0.5 ml staining solution / staining experiment with 1x probe concentration. The number of staining experiments can be further increased by reducing the staining solution volume or the probe concentration.
*Halo-tag™ is a registered trademark of Promega Corporation.
