Product Description
SPY555-CA is the ChloroAlkane (CA) derivative of our SPY555 fluorophore. It emits light in the orange part of the UV-ViS spectrum, it is fluorogenic, highly cell permeable and well suited for STED and SIM superresolution imaging. SPY555-CA can be imaged with a standard Cy3 filterset. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. It has the same structure as MaP555-halo or MaP555-CA, the name used in its original publication in Nature Chemistry1).
ChloroAlkane (CA) is the substrate of the self labeling tag Halo-tag™*. Upon reaction with a CA derivative, Halo-tag™* forms a covalent bond with the substrate. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as Halo-tag™* fusion.
Probe Properties
| Absorbance maximum λabs | 555 nm |
| Fluorescence maximum λfl | 580 nm |
| Works on fixed cells? | Yes, any type of fixation method |
| Probe quantity | 10 nmol |
| Fluorescence lifetime (Halo-tag bound) | 2.3 ns |
| STED depletion wavelength | 660 or 775 nm |
| Shipping | room temperature |
| Storage | -20°C |
1. “A general strategy to develop cell permeable and fluorogenic probes for multicolour nanoscopy”; Lu Wang, et. al.; Nature Chemistry 12, 165-172, 2020.
**Based on the following conditions: 0.5 ml staining solution / staining experiment with 1x probe concentration. The number of staining experiments can be further increased by reducing the staining solution volume or the probe concentration.
*Halo-tag™ is a registered trademark of Promega Corporation.
