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SPY700-tubulin
SiR700 Absorption and Emission Spectra
SPY700-tubulin
SiR700 Absorption and Emission Spectra

SPY700-tubulin

SPY700-tubulin is a bright far-red, fluorogenic, cell permeable and highly specific live cell probe for fluorescence imaging of microtubules.

Product contains 1 vial of SPY700-tubulin in lyophilised form allowing to prepare 50 ul of 1000x DMSO stock solution (100 stainings*).

SKU: SC603 Category: SPY probes
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Product Description

SPY700-tubulin is a bright, far-red & non toxic live cell microtubule stain based on our SPY™ dyes series. Its optimized structure allows quick labeling of microtubules in live cells with high specificity and very low background. SPY700-tubulin stains microtubules in live cells without the need for genetic manipulation or overexpression of fluorescent proteins. SPY700-tubulin enables multicolor imaging with SPY505, SPY555, SPY595, SPY650, SiR, GFP and mcherry. SPY700-tubulin can be imaged with Cy5 filtersets but using a 700 nm LP emission filter is best. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. Probe quantity allows 100 staining experiments.*

Probe Properties

 

Absorbance maximum λabs 696 nm
Fluorescence maximum λfl 718 nm
Works on fixed cells? No
Probe quantity 100 stainings*
Fluorescence lifetime 2.2 ns
STED depletion wavelength 780-820 nm
Shipping room temperature
Storage -20°C

 

*Based on the following conditions: 0.5 ml staining solution / staining experiment with 1x probe concentration. The number of staining experiments can be further increased by reducing the staining solution volume or the probe concentration.

SPY700-tubulin datasheet

SPY700-tubulin SDS

Ex/Em spectra (excel format)

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