Product Description
SPY555-FastAct_X is a bright, orange, fluorogenic & non toxic F-actin probe based on our SPY™ dyes series. Its optimized actin ligand was engineered to avoid F-actin stabilization and fast exchange1). This unique feature renders SPY555-FastAct_X non-toxic and minimally perturbing cellular actin processes. It is able to label highy dynamic actin, just like LifeAct™** but the probe does not require any genetic manipulation, transfection or overexpression of fluorescent proteins. SPY555-FastAct_X is based on a bright, low-phototoxic & photostable SPY555 fluorophore which is far superior than fluorescent proteins and STED compatible.
SPY555-FastAct_X enables multicolor imaging with SPY505, SPY595, SPY650, SPY700, SiR and GFP. SPY555-FastAct_X can be imaged with standard Cy3 filterset. It can be used for widefield, confocal, SIM or STED imaging of living cells and tissue. Probe quantity allows 100 staining experiments.*
Probe Properties
| Absorbance maximum λabs | 564 nm |
| Fluorescence maximum λfl | 589 nm |
| Works on fixed cells? | No |
| Probe quantity | 100 stainings* |
| Fluorescence lifetime | n.d. |
| STED depletion wavelength | 660 nm |
| Shipping | room temperature |
| Storage | -20°C |
1) SiR-XActin: A fluorescent probe for imaging actin dynamics in live cells V. Nasufovic et al.;
*Based on the following conditions: 0.5 ml staining solution / staining experiment with 1x probe concentration. The number of staining experiments can be further increased by reducing the staining solution volume or the probe concentration.
**LifeAct™ is a registered trademark of Ibidi GmbH.
