Product Description
Halo-Flipper is a fluorescent probe that specifically labels Halo-tag™* and reports membrane tension changes through its fluorescence lifetime changes. It contains a ChloroAlkane Halo-tag™* ligand as well as a tethered Flipper-TR fluorophore which senses changes of the organization of lipid bilayer membranes surrounding the Halo-tag™* protein. Halo-Flipper is cell permeable, spontaneously labels Halotag expressing cells and is only fluorescent when inserted in a lipid membrane. It has a broad absorption and emission spectrum, excitation can be commonly performed with a 488nm laser, while emission is collected between 575 and 625nm. It is the perfect tool to precisely localize the Flipper-TR membrane tension fluorophore within cells.
ChloroAlkane (CA) is the substrate of the self labeling tag Halo-tag™*. Upon reaction with a CA derivative, Halo-tag™* forms a covalent bond with the substrate. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as Halo-tag™* fusion.
A detailed protocol for staining and FLIM imaging can be found in the datasheet. Probe quantity allows 100 staining experiments.**
Photophysical properties
λabs 480 nm
λfl 600 nm
εmax 1.66·104 mol-1·cm-1 (in DMSO)
lifetime 2.8-7 ns
QY 30% (in AcOEt)
Downloads:
Flipper-TR® fluorophore Abs/Em spectra (Excel format)
References:
1) Colom A, et al.: A fluorescent membrane tension probe, Nat Chem, 2018, 10:1118–1125 ().
2) Straková K., et al.: HaloFlippers: A General Tool for the Fluorescence Imaging of Precisely Localized Membrane Tension Changes in Living Cells, ACS Cent. Sci. 2020, 6, 8, 1376–1385
*Halo-tag™ is a registered trademark of Promega Corporation.
**Based on the following conditions: 0.5 – 1 ml staining solution / staining experiments with 200 nM probe concentration. The number of staining experiments can be further increased by reducing volume or probe concentration.
Halo-Flipper probe is distributed by Spirochrome under an exclusive license from the University of Geneva, Switzerland and was developed by the NCCR Chemical Biology.
This product is currently not available in the United States of America.
