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STED image of Live HeLa cell stained with SPY555-tubulin (magenta), SPY595-DNA (orange) and SiR-actin (white) with a single 775 nm STED line
Live HeLa cells stained with SPY555-actin (magenta), SPY595-DNA (orange) and SPY650-tubulin (blue)
HeLa cells stained with 1x SPY595-DNA
HeLa cells stained with 1x SPY595-DNA
HeLa cells stained with 1x SPY595-DNA
HeLa cells stained with 1x SPY595-DNA
BJ cells stained with 1x SPY595-DNA
STED image of Live HeLa cell stained with SPY555-tubulin (magenta), SPY595-DNA (orange) and SiR-actin (white) with a single 775 nm STED line
Live HeLa cells stained with SPY555-actin (magenta), SPY595-DNA (orange) and SPY650-tubulin (blue)
HeLa cells stained with 1x SPY595-DNA
HeLa cells stained with 1x SPY595-DNA
HeLa cells stained with 1x SPY595-DNA
HeLa cells stained with 1x SPY595-DNA
BJ cells stained with 1x SPY595-DNA

SPY595-DNA

SPY595-DNA is a red, fluorogenic, non toxic, cell permeable and highly specific live cell DNA probe for fluorescence imaging of the nucleus and DNA.

Product contains 1 vial of SPY595-DNA in lyophilised form allowing to prepare 50 ul of 1000x DMSO stock solution (100 stainings*).


SKU: SC301 Category: SPY probes
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Product Description

SPY595-DNA is a bright, photostable, red & non toxic live cell nuclear stain based on our SPYTM dyes series. Its optimized structure allows quick labeling of DNA in live & fixed cells with high specificity and very low background. SPY595-DNA stains the nuclei of live or fixed cells without the need for genetic manipulation or overexpression of fluorescent proteins. Its emission in the red minimizes phototoxicity and sample autofluorescence. SPY595-DNA enables multicolor imaging with SPY505, SPY555, SPY650, SPY700, SiR and GFP. SPY595-DNA can be imaged with standard Alexa594TM or Texas Red filtersets. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. Probe quantity allows 100 staining experiments.*

Probe Properties

 

Absorbance maximum λabs 599 nm
Fluorescence maximum λfl 615 nm
Works on fixed cells? yes, PFA and methanol
Probe quantity 100 stainings*
Fluorescence lifetime n.d.
STED depletion wavelength 775 nm
Shipping room temperature
Storage -20°C

 

*Based on the following conditions: 0.5 ml staining solution / staining experiment with 1x probe concentration. The number of staining experiments can be further increased by reducing the staining solution volume or the probe concentration.

Latest citing articles

SPY595-DNA datasheet

SPY595-DNA SDS

Ex/Em spectra (excel format)

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