Product Description
CPY-CA is the ChloroAlkane (CA) derivative of the fluorophore carbopyronin (CPY). It emits light in the red part of the UV-ViS spectrum, it is bright, highly cell permeable and well suited for STED and SIM superresolution imaging. CPY-CA can be imaged with 600/20 nm and 640/40 nm Abs/Em settings. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue.
ChloroAlkane (CA) is the substrate of the self labeling tag Halo-tag™*. Upon reaction with a CA derivative, Halo-tag™* forms a covalent bond with the substrate. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as Halo-tag™* fusion.
Probe Properties
| Absorbance maximum λabs | 606 nm |
| Fluorescence maximum λfl | 626 nm |
| Works on fixed cells? | Yes, any type of fixation method |
| Probe quantity | 10 nmol |
| Fluorescence lifetime (Halo-tag bound) | 3.2 ns |
| STED depletion wavelength | 775 nm |
| Shipping | room temperature |
| Storage | -20°C |
**Based on the following conditions: 0.5 ml staining solution / staining experiment with 1x probe concentration. The number of staining experiments can be further increased by reducing the staining solution volume or the probe concentration.
*Halo-tag™ is a registered trademark of Promega Corporation.
