Product Description
BD626-CA (also named BD626-HTL) is a ChloroAlkane (CA) derivative of the bridged-bicycle-strengthened red fluorophore BD615. The unique BriDye™ bicyclic ether bridge technology1) confers to BD626-CA an exceptional photostability. It emits light in the orange part of the UV-ViS spectrum, it is highly cell permeable and well suited for superresolution imaging. BDBD626-CA absorption and emission maxima are 626 nm and 652 nm, respectively with an extinction coefficient of 130 000 M⁻¹ cm⁻¹ and a quantum yield of 0.81 (HT7 bound values). BD626-CA is highly cell-permeable, highly photostable, and compatible with e.g. 600/20 nm and 640/40 nm excitation and emission filters, respectively. BD626-CA is highly suited for widefield, confocal, SIM, or STED imaging in living or fixed specimens.
ChloroAlkane (CA) is the substrate of the self labeling tag Halo-tag™*. Upon reaction with a CA derivative, Halo-tag™* forms a covalent bond with the substrate. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as Halo-tag™* fusion.
Probe Properties
| Absorbance maximum λabs | 626 nm |
| Fluorescence maximum λfl | 652 nm |
| Works on fixed cells? | Yes, any type of fixation method |
| Probe quantity | 10 nmol |
| Fluorescence lifetime (free dye) | 3.7 ns |
| STED wavelength | 775 nm |
| MW | 799.4 g/mol |
| Shipping | room temperature |
| Storage | -20°C |
1. “A palette of bridged bicycle-strengthened fluorophores.”; Zhang, J., Zhang, K., Wang, K. et al. ; Nat Methods 22, 1276–1287 (2025)
**Based on the following conditions: 0.5 ml staining solution / staining experiment with 1x probe concentration. The number of staining experiments can be further increased by reducing the staining solution volume or the probe concentration.
*Halo-tag™ is a registered trademark of Promega Corporation.
BriDye™ products are proposed under a GenVivo Tech partnership
