Product Description
BD566-CA (also named BD566-HTL) is a ChloroAlkane (CA) derivative of the bridged-bicycle-strengthened orange fluorophore BD566. The unique BriDye™ bicyclic ether bridge technology1) confers to BD566-CA an exceptional photostability. It emits light in the orange part of the UV-ViS spectrum, it is highly cell permeable and well suited for superresolution imaging. BD566-CA absorption and emission maxima are 566 nm and 594 nm, respectively with an extinction coefficient of 108 000 M⁻¹ cm⁻¹ and a quantum yield of 0.85 (HT7 bound values). BD566-CA is highly cell-permeable, highly photostable, and compatible with standard Cy3 or TMR filter settings for widefield, confocal, SIM, or STED imaging in living or fixed specimens.
ChloroAlkane (CA) is the substrate of the self labeling tag Halo-tag™*. Upon reaction with a CA derivative, Halo-tag™* forms a covalent bond with the substrate. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as Halo-tag™* fusion.
Probe Properties
| Absorbance maximum λabs | 566 nm |
| Fluorescence maximum λfl | 594 nm |
| Works on fixed cells? | Yes, any type of fixation method |
| Probe quantity | 10 nmol |
| Fluorescence lifetime (free dye) | 3.2 ns |
| MW (free dye) | 772.3 g/mol |
| Shipping | room temperature |
| Storage | -20°C |
1. “A palette of bridged bicycle-strengthened fluorophores.”; Zhang, J., Zhang, K., Wang, K. et al. ; Nat Methods 22, 1276–1287 (2025)
**Based on the following conditions: 0.5 ml staining solution / staining experiment with 1x probe concentration. The number of staining experiments can be further increased by reducing the staining solution volume or the probe concentration.
*Halo-tag™ is a registered trademark of Promega Corporation.
BriDye™ products are proposed under a GenVivo Tech partnership
